The LYSO-ID Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (ENZ-52405), form metachromatic artifacts that interfere with multicolor imaging applications. LYSO-ID Green dye generates emission profiles that can be multiplexed with other fluorophores. The dye accumulates in acidic compartments, such as endosomes, lysosomes, and secretory vesicles. Low micromolar concentrations of LYSO-ID Green dye are sufficient for staining mammalian cells. This has been validated with the human cervical carcinoma cell line, HeLa, the human T-lymphocyte cell line, Jurkat, and the human bone osteosarcoma epithelial cell line, U2OS. The LYSO-ID Green dye is a new green-emitting, cell-permeable small organic probe molecule that spontaneously localizes to live cell acidic organelles. Applications: Fluorescence microscopy, Fluorescent detection .